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  • Featured Product
  • KD/KO Validated

ZAK Polyclonal antibody

ZAK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28761-1-AP

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZAK Polyclonal antibody (28761-1-AP)

    WB analysis using 28761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28761-1-AP (ZAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZAK Polyclonal antibody (28761-1-AP)

    WB analysis of HeLa using 28761-1-AP

    WB result of ZAK antibody (28761-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZAK transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZAK Polyclonal antibody (28761-1-AP)

    IHC staining of human colon cancer using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZAK Polyclonal antibody (28761-1-AP)

    IHC staining of human colon cancer using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse tongue tissue using ZAK Polyclonal antibody (28761-1-AP)

    IHC staining of mouse tongue using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat tongue tissue using ZAK Polyclonal antibody (28761-1-AP)

    IHC staining of rat tongue using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZAK Polyclonal antibody (28761-1-AP)

    IF Staining of HepG2 using 28761-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (28761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue, rat tongue tissue, mouse tongue tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28761-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZAK fusion protein Ag30599 相同性解析による交差性が予測される生物種
Full Name sterile alpha motif and leucine zipper containing kinase AZK
Calculated molecular weight 91 kDa
Observed molecular weight91kDa, 51 kDa
GenBank accession numberBC001401
Gene symbol ZAK
Gene ID (NCBI) 51776
RRIDAB_2918199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Protocols

Product Specific Protocols
WB protocol for ZAK antibody 28761-1-APDownload protocol
IHC protocol for ZAK antibody 28761-1-APDownload protocol
IF protocol for ZAK antibody 28761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genome Biol

The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model

Authors - Yunfei Li
  • KD Validated
View Article