Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MDA-MB-231 cells |
| Positive IHC detected in | human colon cancer tissue, rat tongue tissue, mouse tongue tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
28761-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZAK fusion protein Ag30599 相同性解析による交差性が予測される生物種 |
| Full Name | sterile alpha motif and leucine zipper containing kinase AZK |
| Calculated molecular weight | 91 kDa |
| Observed molecular weight | 91kDa, 51 kDa |
| GenBank accession number | BC001401 |
| Gene Symbol | ZAK |
| Gene ID (NCBI) | 51776 |
| RRID | AB_2918199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZAK antibody 28761-1-AP | Download protocol |
| IHC protocol for ZAK antibody 28761-1-AP | Download protocol |
| IF protocol for ZAK antibody 28761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genome Biol The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model
| ||
J Cell Mol Med Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals |