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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZAK Monoclonal antibody, PBS Only

ZAK Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10E4

Cat no : 68599-1-PBS

Synonyms

AZK, HCCS 4, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis using 68599-1-Ig (same clone as 68599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis of rat skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit skeletal muscle tissue using ZAK Monoclonal antibody (68599-1-Ig)

    WB analysis of rabbit skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

    rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68599-1-PBS targets ZAK in WB, Indirect ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Reactivity Human, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ZAK fusion protein Ag6747 相同性解析による交差性が予測される生物種
Full Name sterile alpha motif and leucine zipper containing kinase AZK
Calculated molecular weight 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC001401
Gene symbol ZAK
Gene ID (NCBI) 51776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.