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  • Featured Product
  • KD/KO Validated

ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15592-1-AP

Synonyms

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of Jurkat using 15592-1-AP

    WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis using 15592-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of Jurkat using 15592-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of A375 using 15592-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    WB analysis of A375 using 15592-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ZAP70 Polyclonal antibody (15592-1-AP)

    IP experiment of Jurkat using 15592-1-AP

    IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human tonsillitis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human lymphoma using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human lymphoma using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human kidney using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human kidney using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human testis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZAP70 Polyclonal antibody (15592-1-AP)

    IHC staining of human testis using 15592-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using ZAP70 Polyclonal antibody (15592-1-AP)

    IF Staining of RAW 264.7 using 15592-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, A375 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15592-1-AP targets ZAP70 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
RRIDAB_2218654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Product Specific Protocols
WB protocol for ZAP70 antibody 15592-1-APDownload protocol
IHC protocol for ZAP70 antibody 15592-1-APDownload protocol
IF protocol for ZAP70 antibody 15592-1-APDownload protocol
IP protocol for ZAP70 antibody 15592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol Methods

An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination

Authors - Jiaqi Zheng
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

hala (Verified Customer) (01-27-2023)

works very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/10000
  • Cell Tissue Type: hepG2
ZAP70 Antibody Western Blot validation (1/10000 dilution) in hepG2 (Cat no:15592-1-AP)