ZAP70 Polyclonal antibody
ZAP70 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 22207-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, RAW 264.7 cells, RAW264.7 |
Positive IP detected in | Jurkat cells |
Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
22207-1-AP targets ZAP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ZAP70 fusion protein Ag17527 相同性解析による交差性が予測される生物種 |
Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
Calculated molecular weight | 619 aa, 70 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC039039 |
Gene symbol | ZAP70 |
Gene ID (NCBI) | 7535 |
RRID | AB_2879029 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ZAP70 antibody 22207-1-AP | Download protocol |
IF protocol for ZAP70 antibody 22207-1-AP | Download protocol |
IP protocol for ZAP70 antibody 22207-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |