Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, RAW 264.7 cells, RAW264.7 |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22207-1-AP targets ZAP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZAP70 fusion protein Ag17527 相同性解析による交差性が予測される生物種 |
| Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
| Calculated molecular weight | 619 aa, 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC039039 |
| Gene Symbol | ZAP70 |
| Gene ID (NCBI) | 7535 |
| ENSEMBL Gene ID | ENSG00000115085 |
| RRID | AB_2879029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZAP70 antibody 22207-1-AP | Download protocol |
| IF protocol for ZAP70 antibody 22207-1-AP | Download protocol |
| IP protocol for ZAP70 antibody 22207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |