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ZAP70 Monoclonal antibody

ZAP70 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B3C6

Cat no : 60200-2-Ig

Synonyms

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-2-Ig)

    WB analysis of Jurkat using 60200-2-Ig

    WB result of ZAP70 antibody (60200-2-Ig, 1:5000) with si-control and si-ZAP70 transfected Jurkat cells..

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-2-Ig)

    WB analysis of Jurkat using 60200-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-2-Ig)

    WB analysis of Jurkat using 60200-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZAP70 Monoclonal antibody (60200-2-Ig)

    WB analysis of Jurkat using 60200-2-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-2-Ig)

    IHC staining of human lymphoma using 60200-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ZAP70 Monoclonal antibody (60200-2-Ig)

    IHC staining of human lymphoma using 60200-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60200-2-Ig is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.