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CoraLite® Plus 488-conjugated ZAP70 Monoclonal antibody
ZAP70 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A11G9
Cat no : CL488-60200
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL488-60200 is ZAP70 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ZAP70 fusion protein Ag7943 相同性解析による交差性が予測される生物種 |
Full Name | zeta-chain (TCR) associated protein kinase 70kDa |
Calculated molecular weight | 70 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC039039 |
Gene symbol | ZAP70 |
Gene ID (NCBI) | 7535 |
RRID | AB_2919236 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 ZAP70 antibody CL488-60200 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |