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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZBTB1 Polyclonal antibody (26287-1-AP)

    WB analysis of HeLa using 26287-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26287-1-AP (ZBTB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZBTB1 Polyclonal antibody (26287-1-AP)

    IP experiment of HeLa using 26287-1-AP

    IP result of anti-ZBTB1 (IP:26287-1-AP, 4ug; Detection:26287-1-AP 1:300) with HeLa cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZBTB1 Polyclonal antibody (26287-1-AP)

    IF Staining of HeLa using 26287-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26287-1-AP (ZBTB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

ZBTB1 Polyclonal antibody

ZBTB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 26287-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

KIAA0997, ZBTB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26287-1-AP targets ZBTB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB1 fusion protein Ag23492 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 1
Observed molecular weight 82 kDa
GenBank accession numberBC050719
Gene Symbol ZBTB1
Gene ID (NCBI) 22890
RRIDAB_2880463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZBTB1 antibody 26287-1-APDownload protocol
IF protocol for ZBTB1 antibody 26287-1-APDownload protocol
IP protocol for ZBTB1 antibody 26287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Authors - Robert T Williams
  • KO Validated
View Article
mouseWB

iScience

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner
View Article