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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using ZBTB10 Polyclonal antibody (22944-1-AP)

    WB analysis of A375 using 22944-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 22944-1-AP (ZBTB10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ZBTB10 Polyclonal antibody (22944-1-AP)

    IP experiment of mouse liver using 22944-1-AP

    IP result of anti-ZBTB10 (IP:22944-1-AP, 3ug; Detection:22944-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

  • Immunoprecipitation (IP) experiment using ZBTB10 Polyclonal antibody (22944-1-AP)

    IP experiment using 22944-1-AP

    IP result of ZBTB10 antibody (22944-1-AP for IP and Detection) with mouse liver tissue lysate.

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ZBTB10 Polyclonal antibody

ZBTB10 Polyclonal Antibody for WB, IP, ELISA
Cat No. 22944-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

RINZF, RINZFC, ZBTB10, Zinc finger protein RIN ZF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22944-1-AP targets ZBTB10 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB10 fusion protein Ag19094 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 10
Calculated molecular weight 871 aa, 95 kDa
Observed molecular weight 65 kDa, 130 kDa
GenBank accession numberBC127098
Gene Symbol ZBTB10
Gene ID (NCBI) 65986
RRIDAB_2879188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96DT7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger and BTB domain-containing protein 10 (ZBTB10), also known as RINZF, RINZFC, May be involved in transcriptional regulation. Multiple isoforms of ZBTB10 exist due to alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for ZBTB10 antibody 22944-1-APDownload protocol
IP protocol for ZBTB10 antibody 22944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols