ZBTB33 Polyclonal antibody
ZBTB33 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 55270-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
55270-1-AP targets ZBTB33 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger and BTB domain containing 33 |
| Calculated molecular weight | 74 kDa |
| Observed molecular weight | 68-74 kDa |
| GenBank accession number | NM_006777 |
| Gene symbol | ZBTB33 |
| Gene ID (NCBI) | 10009 |
| RRID | AB_2881295 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| IHC protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| IP protocol for ZBTB33 antibody 55270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun ZNF131 facilitates the growth of hepatocellular carcinoma by acting as a transcriptional activator of SMC4 expression |
