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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    WB analysis of HEK-293 using 24544-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24544-1-AP (ZBTB8A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    IP experiment of HEK-293 using 24544-1-AP

    IP result of anti-ZBTB8A (IP:24544-1-AP, 3ug; Detection:24544-1-AP 1:300) with HEK-293 cells lysate 920ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    IF Staining of HEK-293 using 24544-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24544-1-AP (ZBTB8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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ZBTB8A Polyclonal antibody

ZBTB8A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24544-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

BOZ F1, BOZF1, ZBTB8, ZBTB8A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24544-1-AP targets ZBTB8A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZBTB8A fusion protein Ag21588 相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 8A
Calculated molecular weight 441 aa, 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC015239
Gene Symbol ZBTB8A
Gene ID (NCBI) 653121
RRIDAB_2879599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BR9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZBTB8A, also named as Zinc finger and BTB domain-containing protein 8A, is a 441 amino acid protein, which may be involved in transcriptional regulation. ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis. The calcualted molecular weight of ZBTB8A is 50 kDa, but modified ZBTB8A is about 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for ZBTB8A antibody 24544-1-APDownload protocol
IF protocol for ZBTB8A antibody 24544-1-APDownload protocol
IP protocol for ZBTB8A antibody 24544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols