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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZC3H11A Recombinant antibody, PBS Only

ZC3H11A Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230191H5

Cat no : 82912-1-PBS

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191H5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZC3H11A Recombinant antibody (82912-1-RR)

    WB analysis using 82912-1-RR (same clone as 82912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82912-1-RR (ZC3H11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82912-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZC3H11A Recombinant antibody (82912-1-RR)

    IHC staining of human ovary cancer using 82912-1-RR (same clone as 82912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82912-1-RR (ZC3H11A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82912-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZC3H11A Recombinant antibody (82912-1-RR)

    IF Staining of HepG2 using 82912-1-RR (same clone as 82912-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZC3H11A antibody (82912-1-RR, Clone: 230191H5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82912-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82912-1-RR

    Affinity and Kinetic Characterization of 82912-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82912-1-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82912-1-PBS targets ZC3H11A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZC3H11A fusion protein Ag23424 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 11A
Observed molecular weight100 kDa
GenBank accession numberBC014268
Gene symbol ZC3H11A
Gene ID (NCBI) 9877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.