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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZC3H11A Recombinant antibody

ZC3H11A Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230191B4

Cat no : 82912-3-RR

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191B4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using ZC3H11A Recombinant antibody (82912-3-RR)

    FC experiment of MCF-7 using 82912-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of A431 cells using ZC3H11A Recombinant antibody (82912-3-RR)

    FC experiment of A431 using 82912-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82912-3-RR

    Affinity and Kinetic Characterization of 82912-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82912-3-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive FC (Intra) detected inA431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82912-3-RR targets ZC3H11A in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZC3H11A fusion protein Ag23424 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 11A
GenBank accession numberBC014268
Gene symbol ZC3H11A
Gene ID (NCBI) 9877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.