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ZC3H12D Polyclonal antibody
ZC3H12D Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 24991-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, K-562 cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
24991-1-AP targets ZC3H12D in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZC3H12D fusion protein Ag21770 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type containing 12D |
| Calculated molecular weight | 527 aa, 58 kDa |
| Observed molecular weight | 55-58 kDa |
| GenBank accession number | BC157832 |
| Gene symbol | ZC3H12D |
| Gene ID (NCBI) | 340152 |
| RRID | AB_2879834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H12D antibody 24991-1-AP | Download protocol |
| IHC protocol for ZC3H12D antibody 24991-1-AP | Download protocol |
| IP protocol for ZC3H12D antibody 24991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Nat Commun Extracellular mRNA transported to the nucleus exerts translation-independent function.
| ||
Comput Struct Biotechnol J Integrating knowledge of protein sequence with protein function for the prediction and validation of new MALT1 substrates | ||
J Biol Chem Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.
| ||
Front Aging Neurosci DNA Methylation Profiling Reveals the Change of Inflammation-Associated ZC3H12D in Leukoaraiosis. | ||
Oncol Lett miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elaine (Verified Customer) (09-06-2024) | Worked very nicely in western blot. Showed one clear band of the correct size in mouse T cell lysate and appears to not have cross-reactivity with Zc3h12a.
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