Validation Data Gallery
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Tested Applications
| Positive WB detected in | Karpas-422 cells, MJ cells, Raji cells, Ramos cells, Daudi cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68847-1-Ig targets ZC3H12D in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ZC3H12D fusion protein Ag21770 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type containing 12D |
| Calculated molecular weight | 527 aa, 58 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC157832 |
| Gene Symbol | ZC3H12D |
| Gene ID (NCBI) | 340152 |
| RRID | AB_3670440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | A2A288 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H12D antibody 68847-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |