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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZC3H8 Monoclonal antibody (68282-1-Ig)

    WB analysis using 68282-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using ZC3H8 Monoclonal antibody (68282-1-Ig)

    WB analysis of LNCaP using 68282-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using ZC3H8 Monoclonal antibody (68282-1-Ig)

    FC experiment of HEK-293 using 68282-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ZC3H8 Monoclonal antibody (68282-1-Ig, Clone:1G8C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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ZC3H8 Monoclonal antibody

ZC3H8 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68282-1-Ig
Clone No.1G8C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68282-1-Ig targets ZC3H8 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZC3H8 fusion protein Ag23496 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 8
Observed molecular weight36 kDa
GenBank accession numberBC032001
Gene Symbol ZC3H8
Gene ID (NCBI) 84524
RRIDAB_2935363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5P1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).

Protocols

Product Specific Protocols
WB protocol for ZC3H8 antibody 68282-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols