Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16820-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16820-1-AP
WB result of ZC3HAV1 antibody (16820-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3HAV1 transfected HEK-293 cells.
WB analysis of HeLa using 16820-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16820-1-AP (ZC3HAV1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16820-1-AP
IP result of anti-ZC3HAV1 (IP:16820-1-AP, 4ug; Detection:16820-1-AP 1:2000) with HeLa cells lysate 1080ug.
IHC staining of human kidney using 16820-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16820-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 16820-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 16820-1-AP (ZC3HAV1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ZC3HAV1 Polyclonal antibody

ZC3HAV1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16820-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

ZAP, PRO1677, PARP13, Inactive Poly [ADP-ribose] polymerase 13, ARTD13

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 28 publications below
IHC	See 2 publications below
IF	See 4 publications below
IP	See 4 publications below
RIP	See 1 publications below

Product Information

16820-1-AP targets ZC3HAV1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ZC3HAV1 fusion protein Ag10364 相同性解析による交差性が予測される生物種
Full Name	zinc finger CCCH-type, antiviral 1
Calculated molecular weight	699aa,78 kDa; 902aa,101 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC025308
Gene Symbol	ZC3HAV1
Gene ID (NCBI)	56829
RRID	AB_2728733
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z2W4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1 antibody 16820-1-AP	Download protocol
IHC protocol for ZC3HAV1 antibody 16820-1-AP	Download protocol
IP protocol for ZC3HAV1 antibody 16820-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CG dinucleotide suppression enables antiviral defence targeting non-self RNA. Authors - Matthew A Takata KD Validated View Article
human	WB	Cell SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Authors - Lea H Gregersen View Article
		Cell Host Microbe HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization. Authors - Charles Bou-Nader View Article
human	WB	Nat Microbiol Rational attenuation of RNA viruses with zinc finger antiviral protein Authors - Daniel Gonçalves-Carneiro View Article
human	WB,IP	Nat Commun The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. Authors - Matthias M Zimmer View Article
human	WB	Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. Authors - Evan P Booy View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16820-1-AP

WB analysis of HEK-293 using 16820-1-AP

WB analysis of HeLa using 16820-1-AP

IP experiment of HeLa using 16820-1-AP

IHC staining of human kidney using 16820-1-AP

IHC staining of human kidney using 16820-1-AP

IHC staining of human stomach cancer using 16820-1-AP

ZC3HAV1 Polyclonal antibody

ZC3HAV1 Polyclonal Antibody for WB, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CG dinucleotide suppression enables antiviral defence targeting non-self RNA.

SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.

HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.

Rational attenuation of RNA viruses with zinc finger antiviral protein

The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.