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ZC3HAV1 Polyclonal antibody
ZC3HAV1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 16820-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 26 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 4 publications below |
| RIP | See 1 publications below |
Product Information
16820-1-AP targets ZC3HAV1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZC3HAV1 fusion protein Ag10364 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type, antiviral 1 |
| Calculated molecular weight | 699aa,78 kDa; 902aa,101 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC025308 |
| Gene symbol | ZC3HAV1 |
| Gene ID (NCBI) | 56829 |
| RRID | AB_2728733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The zinc-finger antiviral protein (ZAP) was originally identifed from a rat cDNA library due to its conferring resistance to retroviruses. ZC3HAV1 is a CCCH-type zinc finger protein, it may primarily function to inhibit viral gene expression and induce an innate immunity to viral infection. Alternative splicing occurs at this locus and two variants, each encoding distinct isoforms. ZC3HAV1 exists some isoforms with MV 101, 77, 67, 42 and 18 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| IHC protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| IP protocol for ZC3HAV1 antibody 16820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature CG dinucleotide suppression enables antiviral defence targeting non-self RNA.
| ||
Cell Host Microbe HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization. | ||
Nat Commun The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting. | ||
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (10-26-2022) | This is a fantastic antibody. It works very reliably at high dilutions.
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