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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZCCHC12 Polyclonal antibody

ZCCHC12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26442-1-AP

Synonyms

SIZN, SIZN1, ZCCHC12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZCCHC12 Polyclonal antibody (26442-1-AP)

    WB analysis using 26442-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26442-1-AP (ZCCHC12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26442-1-AP targets ZCCHC12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZCCHC12 fusion protein Ag23858 相同性解析による交差性が予測される生物種
Full Name zinc finger, CCHC domain containing 12
Observed molecular weight 35-40 kDa
GenBank accession numberBC031241
Gene symbol ZCCHC12
Gene ID (NCBI) 170261
RRIDAB_3085873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger CCHC domain-containing protein 12 (ZCCHC12) functions as a transcriptional coactivator in the bone morphogenetic protein (BMP)-signaling pathway (PMID: 19578717; 19416967). It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex (PMID: 18160706). It contributes to the BMP-induced enhancement of cholinergic-neuron-specific gene expression. A variant in ZCCHC12 may play a role in X-linked cognitive disability (PMID: 18798319).

Protocols

Product Specific Protocols
WB protocol for ZCCHC12 antibody 26442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols