Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, HEK-293T cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 12 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
25196-1-AP targets ZCCHC6 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZCCHC6 fusion protein Ag18240 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, CCHC domain containing 6 |
| Calculated molecular weight | 1495 aa, 171 kDa |
| Observed molecular weight | 171 kDa |
| GenBank accession number | BC032456 |
| Gene Symbol | ZCCHC6 |
| Gene ID (NCBI) | 79670 |
| RRID | AB_2879953 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VYS8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCCHC6 antibody 25196-1-AP | Download protocol |
| IP protocol for ZCCHC6 antibody 25196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Mol Cell 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
| ||
Nat Commun AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2. | ||
Elife The molecular basis of coupling between poly(A)-tail length and translational efficiency. |