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  • KD/KO Validated

ZCCHC6 Polyclonal antibody

ZCCHC6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25196-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ELISA

HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7, TUT7, TUTase 7, ZCCHC6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 650 μg/ml
20 μL, 650 μg/ml
150 μL, 650 μg/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inPC-3 cells, HEK-293T cells, mouse brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25196-1-AP targets ZCCHC6 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZCCHC6 fusion protein Ag18240 相同性解析による交差性が予測される生物種
Full Name zinc finger, CCHC domain containing 6
Calculated molecular weight 1495 aa, 171 kDa
Observed molecular weight 171 kDa
GenBank accession numberBC032456
Gene Symbol ZCCHC6
Gene ID (NCBI) 79670
RRIDAB_2879953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VYS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

Protocols

Product Specific Protocols
WB protocol for ZCCHC6 antibody 25196-1-APDownload protocol
IP protocol for ZCCHC6 antibody 25196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
humanWB

Mol Cell

3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.

Authors - Acong Yang
  • KO Validated
humanWB

Mol Cell

A Mechanism for microRNA Arm Switching Regulated by Uridylation.

Authors - Haedong Kim
  • KD Validated
humanWB,IP

Nat Commun

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

Authors - Acong Yang
humanWB,CoIP

Nat Commun

AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.

Authors - Acong Yang
humanWB

Elife

The molecular basis of coupling between poly(A)-tail length and translational efficiency.

Authors - Kehui Xiang
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