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- KD/KO Validated
ZCCHC8 Polyclonal antibody
ZCCHC8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 23374-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse thymus tissue, HepG2 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
23374-1-AP targets ZCCHC8 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZCCHC8 fusion protein Ag19985 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, CCHC domain containing 8 |
| Calculated molecular weight | 707 aa, 79 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC065918 |
| Gene symbol | ZCCHC8 |
| Gene ID (NCBI) | 55596 |
| RRID | AB_2879269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCCHC8 antibody 23374-1-AP | Download protocol |
| IHC protocol for ZCCHC8 antibody 23374-1-AP | Download protocol |
| IF protocol for ZCCHC8 antibody 23374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Nuclear RNA catabolism controls endogenous retroviruses, gene expression asymmetry, and dedifferentiation
| ||
Nucleic Acids Res mRNAs are sorted for export or degradation before passing through nuclear speckles. | ||
Nucleic Acids Res Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. | ||
Cell Rep Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells.
| ||
Nat Neurosci Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration
| ||
PLoS Genet RBMX involves in telomere stability maintenance by regulating TERRA expression |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Moritz (Verified Customer) (08-20-2024) | I used this antibody to detect ZCCHC8 in HEK293T WT cell lysate on Western blot, which worked perfectly. The signal was very specific, no unspecific bands were detectable.
|
FH Roy (Verified Customer) (06-15-2024) | Perfect for detecting the NEXT complex component ZCCHC8 by WB (1h incubation at room temperature, 1/1000 dilution)
|
