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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZCRB1 Polyclonal antibody

ZCRB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25629-1-AP

Synonyms

Zinc finger CCHC-type and RNA-binding motif-containing protein 1, SNRNP31

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZCRB1 Polyclonal antibody (25629-1-AP)

    WB analysis using 25629-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25629-1-AP (ZCRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZCRB1 Polyclonal antibody (25629-1-AP)

    IHC staining of human ovary cancer using 25629-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25629-1-AP (ZCRB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inARPE-19 cells, Neuro-2a cells, Raji cells, SMMC-7721 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25629-1-AP targets ZCRB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZCRB1 fusion protein Ag22095 相同性解析による交差性が予測される生物種
Full Name zinc finger CCHC-type and RNA binding motif 1
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC022543
Gene symbol ZCRB1
Gene ID (NCBI) 85437
RRIDAB_2880169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZCRB1 antibody 25629-1-APDownload protocol
IHC protocol for ZCRB1 antibody 25629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5.

Authors - Jian Song
View Article
humanWB

bioRxiv

Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling

Authors - Geralle Powell-Rodgers
View Article