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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZDHHC13 Polyclonal antibody

ZDHHC13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24759-1-AP

Synonyms

Huntingtin-interacting protein 14-related protein, HIP3RP, HIP14-related protein, HIP14L, EC:2.3.1.225

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ZDHHC13 Polyclonal antibody (24759-1-AP)

    WB analysis of mouse kidney using 24759-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ZDHHC13 Polyclonal antibody (24759-1-AP)

    WB analysis of mouse testis using 24759-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using ZDHHC13 Polyclonal antibody (24759-1-AP)

    WB analysis of mouse skin using 24759-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZDHHC13 Polyclonal antibody (24759-1-AP)

    WB analysis of HEK-293 using 24759-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using ZDHHC13 Polyclonal antibody (24759-1-AP)

    WB analysis of Neuro-2a using 24759-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24759-1-AP (ZDHHC13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ZDHHC13 Polyclonal antibody (24759-1-AP)

    IHC staining of human stomach using 24759-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ZDHHC13 Polyclonal antibody (24759-1-AP)

    IF Staining of HEK-293 using 24759-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24759-1-AP (ZDHHC13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, mouse skin tissue, mouse testis tissue, Neuro-2a cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24759-1-AP targets ZDHHC13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZDHHC13 fusion protein Ag21607 相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 13
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC056152
Gene symbol ZDHHC13
Gene ID (NCBI) 54503
RRIDAB_2879708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot.

Protocols

Product Specific Protocols
WB protocol for ZDHHC13 antibody 24759-1-APDownload protocol
IHC protocol for ZDHHC13 antibody 24759-1-APDownload protocol
IF protocol for ZDHHC13 antibody 24759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB

J Cell Biol

Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.

Authors - Thaddaeus Mutugi Nthiga
View Article