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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZDHHC15 Polyclonal antibody

ZDHHC15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 21627-1-AP

Synonyms

DHHC 15, MRX91, Palmitoyltransferase ZDHHC15, ZDHHC15

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis using 21627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of HepG2 using 21627-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, SMMC-7721 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

21627-1-AP targets ZDHHC15 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZDHHC15 fusion protein Ag16152 相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 15
Calculated molecular weight 337 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC103982
Gene symbol ZDHHC15
Gene ID (NCBI) 158866
RRIDAB_2878892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZDHHC15 antibody 21627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Mol Cell Endocrinol

ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells

Authors - Shanmeizi Zhao
View Article