Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, mouse brain tissue |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84803-4-RR targets ZDHHC5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ZDHHC5 fusion protein Ag15022 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, DHHC-type containing 5 |
| Calculated molecular weight | 715 aa, 78 kDa |
| Observed molecular weight | 75-77 kDa |
| GenBank accession number | BC026967 |
| Gene Symbol | ZDHHC5 |
| Gene ID (NCBI) | 25921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9C0B5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZDHHC5 antibody 84803-4-RR | Download protocol |
| IP protocol for ZDHHC5 antibody 84803-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |