ZDHHC9 Polyclonal antibody

Name: ZDHHC9 Polyclonal antibody
Brand: Proteintech
SKU: 24046-1-AP

ZDHHC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24046-1-AP

Synonyms

CGI 89, CXorf11, DHHC 9, DHHC9, MMSA 1, Palmitoyltransferase ZDHHC9, UNQ261/PRO298, ZDHHC10, ZDHHC9, Zinc finger protein 379, ZNF379, ZNF380

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 24046-1-AP
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 24046-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24046-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24046-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 24046-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24046-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 24046-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 24046-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 24046-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of SH-SY5Y using 24046-1-AP
Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 24046-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse brain tissue
Positive IHC detected in	human testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

24046-1-AP targets ZDHHC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ZDHHC9 fusion protein Ag20881 相同性解析による交差性が予測される生物種
Full Name	zinc finger, DHHC-type containing 9
Calculated molecular weight	41 kDa
Observed molecular weight	38-48 kDa
GenBank accession number	BC006200
Gene symbol	ZDHHC9
Gene ID (NCBI)	51114
RRID	AB_2879415
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Protocols

Product Specific Protocols
WB protocol for ZDHHC9 antibody 24046-1-AP	Download protocol
IHC protocol for ZDHHC9 antibody 24046-1-AP	Download protocol
IF protocol for ZDHHC9 antibody 24046-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	IHC,IF	Discov Oncol ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance Authors - Chao Xu View Article
human	WB	Cancer Lett Palmitoylation alters LDHA activity and pancreatic cancer response to chemotherapy Authors - Luojun Chen View Article
mouse	WB,IF	Adv Mater Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy Authors - Guoqing Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZDHHC9 Polyclonal antibody

ZDHHC9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 24046-1-AP

WB analysis of SH-SY5Y using 24046-1-AP

WB analysis of mouse liver using 24046-1-AP

WB analysis of mouse brain using 24046-1-AP

WB analysis of mouse skeletal muscle using 24046-1-AP

WB analysis of mouse brain using 24046-1-AP

IHC staining of human testis using 24046-1-AP

IHC staining of human liver using 24046-1-AP

IHC staining of human testis using 24046-1-AP

IF Staining of SH-SY5Y using 24046-1-AP