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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of HeLa using 66279-1-Ig (same clone as 66279-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of Jurkat using 66279-1-Ig (same clone as 66279-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using ZEB1 Monoclonal antibody (66279-1-Ig)

    WB analysis of SKOV-3 using 66279-1-Ig (same clone as 66279-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human lung cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Monoclonal antibody (66279-1-Ig)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

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ZEB1 Monoclonal antibody, PBS Only

ZEB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66279-1-PBS
Clone No.1H1F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66279-1-PBS targets ZEB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZEB1 fusion protein Ag17118 相同性解析による交差性が予測される生物種
Full Name zinc finger E-box binding homeobox 1
Calculated molecular weight 1125 aa, 124 kDa
Observed molecular weight 190-200 kDa
GenBank accession numberBC112392
Gene Symbol ZEB1
Gene ID (NCBI) 6935
RRIDAB_2881662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37275
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa.