Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, rat testis tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16647-1-AP targets ZER1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZER1 fusion protein Ag10031 相同性解析による交差性が予測される生物種 |
| Full Name | zer-1 homolog (C. elegans) |
| Calculated molecular weight | 766 aa, 88 kDa |
| Observed molecular weight | 80-88 kDa |
| GenBank accession number | BC052563 |
| Gene Symbol | ZER1 |
| Gene ID (NCBI) | 10444 |
| RRID | AB_2878294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z7L7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZER1 antibody 16647-1-AP | Download protocol |
| IHC protocol for ZER1 antibody 16647-1-AP | Download protocol |
| IP protocol for ZER1 antibody 16647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |