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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZFHX3 Polyclonal antibody

ZFHX3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20157-1-AP

Synonyms

C16orf47, ATBT, AT-binding transcription factor 1, ATBF1, AT motif-binding factor 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human breast cancer using 20157-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human prostate cancer using 20157-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human prostate cancer using 20157-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human breast cancer using 20157-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZFHX3 Polyclonal antibody (20157-1-AP)

    IF Staining of HeLa using 20157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFHX3 antibody (20157-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZFHX3 Polyclonal antibody (20157-1-AP)

    IF Staining of MCF-7 using 20157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZFHX3 antibody (20157-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20157-1-AP targets ZFHX3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFHX3 fusion protein Ag14080 相同性解析による交差性が予測される生物種
Full Name zinc finger homeobox 3
Calculated molecular weight 3703 aa, 404 kDa
GenBank accession numberBC029653
Gene symbol ZFHX3
Gene ID (NCBI) 463
RRIDAB_3669324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc finger homeobox protein 3 (ZFHX3) encodes a transcription factor which contains four homeodomains and seventeen zinc fingers. The ZFHX3 transcription factor appears to regulate myogenic and neuronal differentiation. Although the function of ZFHX3 in cardiac tissue is unknown, it is expressed in mouse hearts. (PMID: 26267381)

Protocols

Product Specific Protocols
IHC protocol for ZFHX3 antibody 20157-1-APDownload protocol
IF protocol for ZFHX3 antibody 20157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols