Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22056-1-AP targets ZFP161 / ZBTB14 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZFP161 / ZBTB14 fusion protein Ag17082 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 161 homolog (mouse) |
| Calculated molecular weight | 449 aa, 51 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC110519 |
| Gene Symbol | ZFP161 |
| Gene ID (NCBI) | 7541 |
| RRID | AB_3085683 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43829 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. The calculated MW of ZFP161 is 52 kDa, 22056-1-AP can detect a band around 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZFP161 / ZBTB14 antibody 22056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |