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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFP161 Recombinant antibody (83909-4-RR)

    WB analysis using 83909-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR)

    FC experiment of MCF-7 using 83909-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83909-4-RR

    Affinity and Kinetic Characterization of 83909-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

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ZFP161 / ZBTB14 Recombinant antibody

ZFP161 / ZBTB14 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83909-4-RR
Clone No.240853B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, L02 cells, Daudi cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83909-4-RR targets ZFP161 / ZBTB14 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZFP161 / ZBTB14 fusion protein Ag16125 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 161 homolog (mouse)
Calculated molecular weight 449 aa, 51 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC110519
Gene Symbol ZFP161
Gene ID (NCBI) 7541
RRIDAB_3671490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43829
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.

Protocols

Product Specific Protocols
WB protocol for ZFP161 / ZBTB14 antibody 83909-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols