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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZFP36L2 Polyclonal antibody

ZFP36L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19005-1-AP

Synonyms

ERF2, ERF 2, EGF-response factor 2, EGF response factor 2, Butyrate response factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZFP36L2 Polyclonal antibody (19005-1-AP)

    WB analysis of HepG2 using 19005-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19005-1-AP (ZFP36L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ZFP36L2 Polyclonal antibody (19005-1-AP)

    WB analysis of HL-60 using 19005-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19005-1-AP (ZFP36L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ZFP36L2 Polyclonal antibody (19005-1-AP)

    IHC staining of human skin cancer using 19005-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZFP36L2 Polyclonal antibody (19005-1-AP)

    IHC staining of human pancreas cancer using 19005-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZFP36L2 Polyclonal antibody (19005-1-AP)

    IHC staining of human pancreas cancer using 19005-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HL-60 cells
Positive IHC detected inhuman skin cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

19005-1-AP targets ZFP36L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFP36L2 fusion protein Ag13518 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 2
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa, 60 kDa
GenBank accession numberBC005010
Gene symbol ZFP36L2
Gene ID (NCBI) 678
RRIDAB_10598773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZFP36L2 antibody 19005-1-APDownload protocol
IHC protocol for ZFP36L2 antibody 19005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

High expression of ZFP36L2 correlates with the prognosis and immune infiltration in lower-grade glioma.

Authors - Min Zhou
View Article
zebrafishWB

J Hum Genet

Genetic association and functional validation of ZFP36L2 in non-syndromic orofacial cleft subtypes

Authors - Jialin Sun
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Zinc finger protein 36 like 2-histone deacetylase 1 axis is involved in the bone responses to mechanical stress

Authors - Bin Wang
View Article