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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85154-4-RR

    Affinity and Kinetic Characterization of 85154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

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ZFP36L2 Recombinant antibody, PBS Only

ZFP36L2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85154-4-PBS
Clone No.242776C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, Indirect ELISA

Synonyms

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85154-4-PBS targets ZFP36L2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZFP36L2 fusion protein Ag13515 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 2
Calculated molecular weight 51 kDa
Observed molecular weight60 kDa
GenBank accession numberBC005010
Gene Symbol ZFP36L2
Gene ID (NCBI) 678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47974
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).