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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZFPM1 Polyclonal antibody

ZFPM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28012-1-AP

Synonyms

FOG, FOG 1, FOG1, Friend of GATA 1, Friend of GATA protein 1, ZFPM1, Zinc finger protein ZFPM1, ZNF408, ZNF89A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    WB analysis of mouse liver using 28012-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZFPM1 Polyclonal antibody (28012-1-AP)

    WB analysis using 28012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    IHC staining of human prostate cancer using 28012-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    IHC staining of human prostate cancer using 28012-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28012-1-AP targets ZFPM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFPM1 fusion protein Ag27829 相同性解析による交差性が予測される生物種
Full Name zinc finger protein, multitype 1
Calculated molecular weight 105 aa
Observed molecular weight105 kDa
GenBank accession numberNM_153813
Gene symbol ZFPM1
Gene ID (NCBI) 161882
RRIDAB_3086021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).

Protocols

Product Specific Protocols
WB protocol for ZFPM1 antibody 28012-1-APDownload protocol
IHC protocol for ZFPM1 antibody 28012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols