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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFR Polyclonal antibody (23174-1-AP)

    WB analysis using 23174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23174-1-AP (ZFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ZFR Polyclonal antibody (23174-1-AP)

    IHC staining of human heart using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ZFR Polyclonal antibody (23174-1-AP)

    IHC staining of human small intestine using 23174-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23174-1-AP (ZFR Antibody) at dilution of 1:50 (under 10x lens).

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ZFR Polyclonal antibody

ZFR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23174-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Zinc finger RNA-binding protein, ZFR1, M-phase phosphoprotein homolog, M phase phosphoprotein homolog, hZFR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23174-1-AP targets ZFR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFR fusion protein Ag19559 相同性解析による交差性が予測される生物種
Full Name zinc finger RNA binding protein
Calculated molecular weight 1074 aa, 117 kDa
Observed molecular weight120 kDa
GenBank accession numberBC137085
Gene Symbol ZFR
Gene ID (NCBI) 51663
RRIDAB_3085708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96KR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZFR antibody 23174-1-APDownload protocol
IHC protocol for ZFR antibody 23174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols