Validation Data Gallery

Filter: AllWBIHC

WB analysis of K-562 using 22218-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 22218-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22218-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 22218-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 22218-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 22218-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 22218-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

Rabenosyn 5 Polyclonal antibody

Rabenosyn 5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22218-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Synonyms

110 kDa protein, Rabenosyn 5, ZFYVE20

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, A549 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue
Positive IHC detected in	human testis tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

22218-1-AP targets Rabenosyn 5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Rabenosyn 5 fusion protein Ag17554 相同性解析による交差性が予測される生物種
Full Name	zinc finger, FYVE domain containing 20
Calculated molecular weight	784 aa, 89 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC106940
Gene Symbol	Rabenosyn 5
Gene ID (NCBI)	64145
RRID	AB_11182179
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1K0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Rabenosyn 5 antibody 22218-1-AP	Download protocol
IHC protocol for Rabenosyn 5 antibody 22218-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. Authors - Ye Chen View Article
human	WB, IHC	J Mol Biol Identification of New Interactions between Endolysosomal Tethering Factors. Authors - Zsófia Simon-Vecsei View Article
mouse	WB,IF,IP	Int J Mol Sci Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage Authors - Masato Mashimo View Article
human	WB,IF	Nat Commun Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic Authors - Seiichi Koike View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 22218-1-AP

WB analysis of mouse heart using 22218-1-AP

WB analysis of mouse brain using 22218-1-AP

WB analysis of A549 using 22218-1-AP

WB analysis of mouse skeletal muscle using 22218-1-AP

WB analysis of mouse kidney using 22218-1-AP

WB analysis of mouse testis using 22218-1-AP

IHC staining of human testis using 22218-1-AP

IHC staining of human testis using 22218-1-AP

IHC staining of human heart using 22218-1-AP