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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using ZFYVE26 Polyclonal antibody (55418-1-AP)

    IHC staining of mouse heart using 55418-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55418-1-AP (ZFYVE26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ZFYVE26 Polyclonal antibody (55418-1-AP)

    IF Staining of NIH/3T3 using 55418-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ZFYVE26 antibody (55418-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZFYVE26 Polyclonal antibody

ZFYVE26 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 55418-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

KIAA0321, Spastizin, SPG15, ZFYVE26

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55418-1-AP targets ZFYVE26 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name zinc finger, FYVE domain containing 26
Calculated molecular weight 285 kDa
GenBank accession numberNM_015346
Gene Symbol ZFYVE26
Gene ID (NCBI) 23503
RRIDAB_3670251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68DK2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFYVE26 is a large protein of 2539 amino acid residues and contains a FYVE domain that confers to the protein binding affinity to the membrane-associated phosphatidylinositol 3-phosphate (PtdIns3P). ZFYVE26 has a diffuse cytoplasmic distribution and partially colocalizes with early endosomes, the endoplasmic reticulum, microtubules, and vesicles. It localizes also to the midbody during cytokinesis (PMID: 24284334).

Protocols

Product Specific Protocols
IHC protocol for ZFYVE26 antibody 55418-1-APDownload protocol
IF protocol for ZFYVE26 antibody 55418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols