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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFYVE28 Polyclonal antibody (25360-1-AP)

    WB analysis using 25360-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25360-1-AP (ZFYVE28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZFYVE28 Polyclonal antibody (25360-1-AP)

    IF Staining of HeLa using 25360-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFYVE28 Polyclonal antibody (25360-1-AP)

    IF Staining of HepG2 using 25360-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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ZFYVE28 Polyclonal antibody

ZFYVE28 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25360-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE28 fusion protein Ag19796 相同性解析による交差性が予測される生物種
Full Name zinc finger, FYVE domain containing 28
Calculated molecular weight 887 aa, 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberBC137309
Gene Symbol ZFYVE28
Gene ID (NCBI) 57732
RRIDAB_3085790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.

Protocols

Product Specific Protocols
WB protocol for ZFYVE28 antibody 25360-1-APDownload protocol
IF protocol for ZFYVE28 antibody 25360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols