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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZG16 Polyclonal antibody

ZG16 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17397-1-AP

Synonyms

Secretory lectin ZG16, JCLN1, JCLN, hZG16, FLJ92276

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis using 17397-1-AP

    mouse colon tissue lysates were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse small intestine using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human colon cancer using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IF Staining of mouse small intestine using 17397-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman colon tissue, mouse colon tissue
Positive IHC detected inmouse pancreas tissue, human colon cancer tissue, human pancreas tissue, mouse colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17397-1-AP targets ZG16 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZG16 fusion protein Ag11332 相同性解析による交差性が予測される生物種
Full Name zymogen granule protein 16 homolog (rat)
Calculated molecular weight 167 aa, 18 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC029149
Gene symbol ZG16
Gene ID (NCBI) 653808
RRIDAB_1939810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)

Protocols

Product Specific Protocols
WB protocol for ZG16 antibody 17397-1-APDownload protocol
IHC protocol for ZG16 antibody 17397-1-APDownload protocol
IF protocol for ZG16 antibody 17397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease

Authors - Tae-Yoon Park
View Article
human,mouseWB,IF

Adv Sci (Weinh)

Gut Dysbiosis Drives Inflammatory Bowel Disease Through the CCL4L2-VSIR Axis in Glycogen Storage Disease

Authors - Jiaoli Lan
View Article