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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZG16 Polyclonal antibody, PBS Only

ZG16 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17397-1-PBS

Synonyms

Secretory lectin ZG16, JCLN1, JCLN, hZG16, FLJ92276

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP (same clone as 17397-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP (same clone as 17397-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis using 17397-1-AP (same clone as 17397-1-PBS)

    mouse colon tissue lysates were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse small intestine using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human colon cancer using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP (same clone as 17397-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IF Staining of mouse small intestine using 17397-1-AP (same clone as 17397-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17397-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17397-1-PBS targets ZG16 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZG16 fusion protein Ag11332 相同性解析による交差性が予測される生物種
Full Name zymogen granule protein 16 homolog (rat)
Calculated molecular weight 167 aa, 18 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC029149
Gene symbol ZG16
Gene ID (NCBI) 653808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)