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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ZGPAT Polyclonal antibody (23203-1-AP)

    WB analysis of L02 using 23203-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZGPAT Polyclonal antibody (23203-1-AP)

    WB analysis of HepG2 using 23203-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ZGPAT Polyclonal antibody

ZGPAT Polyclonal Antibody for WB, ELISA
Cat No. 23203-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZC3HDC9, ZC3H9, KIAA1847, GPATCH6, GPATC6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, HepG2 cells,

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23203-1-AP targets ZGPAT in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZGPAT fusion protein Ag19620 相同性解析による交差性が予測される生物種
Full Name zinc finger, CCCH-type with G patch domain
Calculated molecular weight 531 aa, 57 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC032612
Gene Symbol ZGPAT
Gene ID (NCBI) 84619
RRIDAB_2879231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N5A5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZGPAT (Zinc finger CCCH-type with G patch domain-containing protein), also known as zinc finger CCCH domain-containing protein 9 (ZC3HDC9) and G patch domain-containing protein 6 (GPATC6), is a 531 amino acid protein that contains a G-patch domain, which is typically found within RNA-binding proteins. Proteins that contain the G-patch domain include some tumor suppressor and DNA-damage repair proteins. ZGPAT also contains one C3H1-type zinc finger, which further supports its probable role as an RNA-binding protein. The gene encoding ZGPAT is inactivated via differential methylation in a oligodendroglioma cell line, suggesting that ZGPAT may have utility as a biomarker. There are several isoforms of ZGPAT that are produced as a result of alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for ZGPAT antibody 23203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols