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- KD/KO Validated
ZHX2 Polyclonal antibody
ZHX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 20136-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse ovary tissue, mouse lung tissue, rat brain tissue, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
20136-1-AP targets ZHX2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZHX2 fusion protein Ag14088 相同性解析による交差性が予測される生物種 |
| Full Name | zinc fingers and homeoboxes 2 |
| Calculated molecular weight | 837 aa, 92 kDa |
| Observed molecular weight | 92-100 kDa |
| GenBank accession number | BC042145 |
| Gene symbol | ZHX2 |
| Gene ID (NCBI) | 22882 |
| RRID | AB_10666438 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IHC protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IF protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IP protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation. | ||
J Pathol ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p. | ||
J Immunol Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.
| ||
Cancer Cell Int ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.
| ||
Biochem Biophys Res Commun Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.
| ||
FEBS Open Bio Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer. |
