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  • Featured Product
  • KD/KO Validated

ZHX2 Monoclonal antibody, PBS Only

ZHX2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E12F2

Cat no : 68268-1-PBS

Synonyms

RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1, AFP regulator 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZHX2 Monoclonal antibody (68268-1-Ig)

    WB analysis using 68268-1-Ig (same clone as 68268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68268-1-Ig (ZHX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZHX2 Monoclonal antibody (68268-1-Ig)

    WB analysis of HEK-293 using 68268-1-Ig (same clone as 68268-1-PBS)

    WB result of ZHX2 antibody (68268-1-Ig; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ZHX2 Monoclonal antibody (68268-1-Ig)

    IF Staining of A431 using 68268-1-Ig (same clone as 68268-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZHX2 antibody (68268-1-Ig, Clone: 4E12F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68268-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68268-1-PBS targets ZHX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZHX2 fusion protein Ag14420 相同性解析による交差性が予測される生物種
Full Name zinc fingers and homeoboxes 2
Calculated molecular weight 837 aa, 92 kDa
Observed molecular weight92-100 kDa
GenBank accession numberBC042145
Gene symbol ZHX2
Gene ID (NCBI) 22882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.