• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZIC1 Polyclonal antibody

ZIC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25004-1-AP

Synonyms

ZIC, ZIC1, Zinc finger protein ZIC 1, ZNF201

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZIC1 Polyclonal antibody (25004-1-AP)

    WB analysis using 25004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25004-1-AP (ZIC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using ZIC1 Polyclonal antibody (25004-1-AP)

    WB analysis of Y79 using 25004-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25004-1-AP (ZIC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inROS1728 cells, Y79 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25004-1-AP targets ZIC1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZIC1 fusion protein Ag13400 相同性解析による交差性が予測される生物種
Full Name Zic family member 1 (odd-paired homolog, Drosophila)
Calculated molecular weight 447 aa, 48 kDa
Observed molecular weight~50 kDa
GenBank accession numberBC104848
Gene symbol ZIC1
Gene ID (NCBI) 7545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZIC1 is a member of the Zinc finger of the cerebellum (ZIC) protein family. In vertebrates, the ZIC gene family plays an important role in neural crest and subsequent cerebellar development. Mutations in ZIC family members have demonstrated that they are essential for proper development of the neural tube, left-right symmetry, and muscle and skeletal development. It has been reported that ZIC1 participates in the regulation of cell proliferation in the dorsal neural tube by inhibition of neural differentiation. Furthermore, ZIC1 is potentially involved in the oncogenesis of undifferentiated nervous system tumors. (PMID:17936758)

Protocols

Product Specific Protocols
WB protocol for ZIC1 antibody 25004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols