Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
51021-1-AP targets Phospho-ZIPK (Ser311) in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | death-associated protein kinase 3 |
| Calculated molecular weight | 52.5 kDa |
| Observed molecular weight | 52.5 kDa |
| GenBank accession number | BC126430 |
| Gene Symbol | DAPK3 |
| Gene ID (NCBI) | 1613 |
| RRID | AB_614997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43293 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DAPK3(Death-associated protein kinase 3) is also named ZIPK(Zipper-interacting protein kinase), and Dlk (DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes(PMID:20038585). It has 2 isoforms produced by alternative splicing, named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IHC protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IP protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal Phosphorylation-dependent control of ZIPK nuclear import is species specific. |