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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZMPSTE24 Polyclonal antibody (12847-1-AP)

    WB analysis of PC-3 using 12847-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using ZMPSTE24 Polyclonal antibody (12847-1-AP)

    IP experiment of PC-3 using 12847-1-AP

    IP result of anti-ZMPSTE24 (IP:12847-1-AP, 4ug; Detection:12847-1-AP 1:300) with PC-3 cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ZMPSTE24 Polyclonal antibody (12847-1-AP)

    IF Staining of PC-3 using 12847-1-AP

    Immunofluorescent analysis of PC-3 cells using 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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ZMPSTE24 Polyclonal antibody

ZMPSTE24 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12847-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Farnesylated proteins-converting enzyme 1, FACE-1, FACE1, FACE 1, EC:3.4.24.84

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12847-1-AP targets ZMPSTE24 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZMPSTE24 fusion protein Ag3949 相同性解析による交差性が予測される生物種
Full Name zinc metallopeptidase (STE24 homolog, S. cerevisiae)
Calculated molecular weight 475 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC037283
Gene Symbol ZMPSTE24
Gene ID (NCBI) 10269
RRIDAB_2257430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

Protocols

Product Specific Protocols
WB protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
IF protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
IP protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols