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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF101 Polyclonal antibody

ZNF101 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25599-1-AP

Synonyms

HZF12

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF101 Polyclonal antibody (25599-1-AP)

    WB analysis of HepG2 using 25599-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25599-1-AP (ZNF101 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF101 Polyclonal antibody (25599-1-AP)

    WB analysis of HeLa using 25599-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25599-1-AP (ZNF101 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ZNF101 Polyclonal antibody (25599-1-AP)

    IHC staining of human stomach cancer using 25599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25599-1-AP (ZNF101 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF101 Polyclonal antibody (25599-1-AP)

    IF Staining of HeLa using 25599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF101 antibody (25599-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25599-1-AP targets ZNF101 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF101 fusion protein Ag22390 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 101
Calculated molecular weight 436 aa, 50 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC119680
Gene symbol ZNF101
Gene ID (NCBI) 94039
RRIDAB_2880152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF101 antibody 25599-1-APDownload protocol
IHC protocol for ZNF101 antibody 25599-1-APDownload protocol
IF protocol for ZNF101 antibody 25599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols