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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF124 Polyclonal antibody

ZNF124 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25216-1-AP

Synonyms

HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF124 Polyclonal antibody (25216-1-AP)

    WB analysis of SH-SY5Y using 25216-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using ZNF124 Polyclonal antibody (25216-1-AP)

    WB analysis of U-937 using 25216-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using ZNF124 Polyclonal antibody (25216-1-AP)

    IP experiment of SH-SY5Y using 25216-1-AP

    IP result of anti-ZNF124 (IP:25216-1-AP, 4ug; Detection:25216-1-AP 1:2000) with SH-SY5Y cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ZNF124 Polyclonal antibody (25216-1-AP)

    IHC staining of human gliomas using 25216-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ZNF124 Polyclonal antibody (25216-1-AP)

    IHC staining of human gliomas using 25216-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF124 Polyclonal antibody (25216-1-AP)

    IF Staining of HepG2 using 25216-1-AP

    Immunofluorescent analysis of HepG2 cells using 25216-1-AP (ZNF124 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSH-SY5Y cells, U-937 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25216-1-AP is ZNF124 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF124 fusion protein Ag17165 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 124
Calculated molecular weight 351 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC099661
Gene symbol ZNF124
Gene ID (NCBI) 7678
RRIDAB_2879965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF124 antibody 25216-1-APDownload protocol
IHC protocol for ZNF124 antibody 25216-1-APDownload protocol
IF protocol for ZNF124 antibody 25216-1-APDownload protocol
IP protocol for ZNF124 antibody 25216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols