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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF148 Polyclonal antibody (31496-1-AP)

    WB analysis using 31496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31496-1-AP (ZNF148 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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ZNF148 Polyclonal antibody

ZNF148 Polyclonal Antibody for WB, ELISA
Cat No. 31496-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZBP 89, Transcription factor ZBP 89, pHZ 52, HT BETA, BFCOL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31496-1-AP targets ZNF148 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF148 fusion protein Ag35742 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 148
Observed molecular weight103 kDa
GenBank accession numberBC035591
Gene Symbol ZNF148
Gene ID (NCBI) 7707
RRIDAB_3670008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UQR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.

Protocols

Product Specific Protocols
WB protocol for ZNF148 antibody 31496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols