Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, T-47D cells, HCT 116 cells, HeLa cells, MDA-MB-231 cells, HepG2 cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84338-7-RR targets ZNF148 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ZNF148 fusion protein Ag35742 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 148 |
| Observed molecular weight | 103 kDa |
| GenBank accession number | BC035591 |
| Gene Symbol | ZNF148 |
| Gene ID (NCBI) | 7707 |
| RRID | AB_3671879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9UQR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Kruppel-like zinc finger protein 148 (ZNF148), also known as β enolase repressor factor 1 (BERF1, ZBP-89, and BFCOL1), encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. ZNF148 participates in the regulation of cell proliferation and death, and is expressed at lower levels in mammalian somatic cells, and highly expressed in tumor cells. Previous studies show that there was a significant correlation between ZNF148 expression and the invasion and metastasis of colorectal tumors. ZNF148 has two alternative splicing isoforms, which are ZNF148FL and ZNF148ΔN. ZNF148FL contains a complete 794 amino acids, ZNF148ΔN lacks the amino-terminal 129 amino acids, and the mechanisms involved in the generation of these two splicing isoforms are not yet clear (PMID: 30463804). Thus, ZNF148 can be observed to have two molecular weights near 103 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF148 antibody 84338-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |