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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF154 Polyclonal antibody

ZNF154 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22161-1-AP

Synonyms

KIAA2003, pHZ 92, zinc finger protein 154, ZNF154

Validation Data Gallery

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  • Western Blot (WB) analysis of COS-7 cells using ZNF154 Polyclonal antibody (22161-1-AP)

    WB analysis of COS-7 using 22161-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZNF154 Polyclonal antibody (22161-1-AP)

    WB analysis of HEK-293 using 22161-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZNF154 Polyclonal antibody (22161-1-AP)

    WB analysis of MCF-7 using 22161-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ZNF154 Polyclonal antibody (22161-1-AP)

    WB analysis of A549 using 22161-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22161-1-AP (ZNF154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCOS-7 cells, A549 cells, HEK-293 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22161-1-AP targets ZNF154 in WB, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF154 fusion protein Ag17812 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 154
Calculated molecular weight 437 aa, 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC152404
Gene symbol ZNF154
Gene ID (NCBI) 7710
RRIDAB_2879012
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sites that display recurrent, aberrant DNA methylation in cancer represent potential biomarkers for screening and diagnostics. Previously, The hypermethylation at the ZNF154 CpG island in 15 solid epithelial tumor types from 13 different organs. The magnitude and reproducibility of the ZNF154 hypermethylation signal across five solid tumor types reinforces the potential of this site as a biomarker for circulating tumor DNA (ctDNA)(PMID: 26857064 ). The calculated molecular weight of ZNF154 is 50 kDa, but the modified ZNF154 protein is about 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for ZNF154 antibody 22161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols