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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF174 Polyclonal antibody

ZNF174 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18157-1-AP

Synonyms

AW 1, zinc finger protein 174, ZNF174, ZSCAN8

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using ZNF174 Polyclonal antibody (18157-1-AP)

    WB analysis of BxPC-3 using 18157-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZNF174 Polyclonal antibody (18157-1-AP)

    WB analysis using 18157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human testis using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human testis using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human skin using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human skin using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human brain using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human brain using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human spleen using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human spleen using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human lung using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human lung using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human ovary using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human ovary using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human heart using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human heart using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human kidney using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human kidney using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human placenta using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZNF174 Polyclonal antibody (18157-1-AP)

    IHC staining of human placenta using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF174 Polyclonal antibody (18157-1-AP)

    IF Staining of HeLa using 18157-1-AP

    Immunofluorescent analysis of Hela cells, using ZNF174 antibody 18157-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18157-1-AP targets ZNF174 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF174 fusion protein Ag12963 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 174
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC000876
Gene symbol ZNF174
Gene ID (NCBI) 7727
RRIDAB_2218459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF174 antibody 18157-1-APDownload protocol
IHC protocol for ZNF174 antibody 18157-1-APDownload protocol
IF protocol for ZNF174 antibody 18157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols